First Report of a Successful Development of Yam Hybrids (Dioscorea alata L.) from Lyophilized and Long-Term Stored Pollens

Various biological constraints including erratic and asynchronous flowering between male female plants hinder successful hybrid development genetic gains in greater yam breeding programs. Therefore, pollen storage has gained much attention to facilitate artificial pollinations increase the gains. This 4-year study aimed at developing a practical long-term technique for of hybrids. Fresh pollens were collected from two Dioscorea alata males, then lyophilized (two lyophilization treatments applied), followed by room temperature (24°C–25°C) 12 months. Moreover, stored tested viability crossing with four varieties. Our results showed that is effective achieving viable after months storage. Treatment 1 (48 h drying) higher germination fertility rates than 2 (72 drying). Although we observed reduction storage, generated seedlings success 12% 21% compared 21%–31% when using fresh pollens. Paternity testing based on molecular genotyping confirmed status obtained seedlings, which grew well greenhouse. Lyophilization approach samples. protocol will positively impact programs particularly countries.